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Theoretical and practical introduction to the molecular analysis of microbial communities in the environment
Whole course but not the seminars
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Theory & Computer Exercises :
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The "molecular revolution" in microbial ecology; microbial diversity; the rRNA approach
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Recent strategies for the molecular identification and quantification of microbes and their activities in nature
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Work with gene databases, clone libraries, and genetic fingerprints
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Literature and web search for gene probes; probe design/evaluation
Laboratory Exercises :
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Analysis of a microbial community: DNA extraction, PCR, cloning, sequence analysis
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Fluorescence in situ hybridization (FISH, CARD-FISH)
· Genetic fingerprinting
Oral Presentation :
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Research paper presentation (individual)
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Group presentation of lab results
Seminar Series with
invited speakers (optional):
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"
Hot topics" in molecular microbial ecology research
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After successful participation in the course, students are able to:
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Perform molecular analyses (PCR, cloning, sequence comparison, FISH, genetic fingerprinting) of microorganisms in environmental samples
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Identify and quantify key organisms of microbial communities
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Explain weaknesses and strengths of the different molecular and microbiological analyses
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Apply the concepts of molecular microbial ecology to problems in microbial ecology
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Andreas Schramm
2 x 4 h course (lecture & lab exercises) plus 1 seminar per week for 7 weeks
EnglishBrock Biology of Microorganisms, 11th ed. 2006, Pearson Prentice Hall.
Lecture notes and selectd research articles
Exam: 2nd quarter
Re-exam: Arranged with lecturer
